Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia

ABSTRACT Soybean crops grown in the plains of eastern Colombia have been affected by incidence rust. This disease, still officially regarded as American soybean rust (caused Phakopsora meibomiae) Colombian Department Agriculture (ICA), causes serious damage to growing areas. Symptoms rust, such reddish-brown lesions, observed since 2004 upper half plants during vegetative stages. In 2005 and 2018, infected leaf tissues uredinospores were collected from an experimental area commercial fields. Once morphological identification Koch’s postulates confirmed presence spp., molecular characterization was performed identify pathogen associated with disease species level. based on standard procedures using internal transcribed spacer (ITS) regions rDNA PCR amplification specific primers for meibomiae P. pachyrhizi. The obtained sequences BLASTed against GenBank/NCBI data bank. Results indicated that pachyrhizi Sydow is fact causative agent Colombia, considering samples 2018. ITS-rDNA deposited at GenBank under accession numbers MK933723 MK933731. finding reported ICA, so they could update phytosanitary status this Colombia.